End-point PCR

Sensitive diagnostic method using specific primers for the detection of RNA or DNA containing pathogens. The detection of the amplification products is done by agarose gel-electrophoresis.

When to use End-Point PCR

  • Routine screening of plant material
  • Confirmation of results from serological methods
  • Diagnostic method with good confidence in negative samples

Advantages and Limitation

 

        • Specific and sensitive, ready-to-use kits, fast and cost effective
        • Optimized and evaluated conditions for the amplification reaction
        • Shipping at room temperature –  no dry ice needed!
        • Laboratoriy equipment needed
        • Agarose Gelelectrophoresis step necessary
        • Experienced operator needed
Molecular methods gain more and more importance in plant pathogen diagnostics. The PCR technique allows specific and sensitive detection of plant pathogens, when serological reagents are not available or a second method is needed, as often demanded in testing schemes by Plant Protection Organizations, especially for quarantine pests. 

DNA PCR Sets

  • Premix (Primers, dNTPs)
  • Positive Control (DNA based)
  • Negative Control (DNA based)

DNA Complete Reaction Kits 

  • Premix (Primers, dNTPs)
  • DNA-Polymerase
  • Reaction Buffer
  • PCR-grade Water
  • Positive Control (DNA based)
  • Negative Control (DNA based)

RNA PCR Complete Reaction Kits (ONE-Tube ONE-Step) 

The reaction is carried out in one tube starting with the reverse transcription of plant virus RNA followed by cDNA amplification. The resulting amplicon is then visualized on an agarose gel. 
  • Premix (Primers, dNTPs)
  • DNA-Polymerase
  • Reverse Transcriptase: M-MLV, RNase H-
  • Reaction Buffer
  • PCR-grade Water
  • Dithiothreitol (DTT)
  • Positive Control (DNA based)
  • Negative Control (DNA based)